Table 2 Nucleotide sequences of primers and oligonucleotides used in vector construction
From: A cell engineering approach to enzyme-based fed-batch fermentation
- Restriction sites indicated by underlined bold (CATATG = NdeI, CTCGAG = XhoI, TCTAGA = XbaI, GGATCC = BamHI, ACTAGT = SpeI) Signal sequence dashed underlined, and ribosome binding site green underlined. The DsbAss upper and DsbAss lower are long oligos designed to anneal to one another and create a double stranded DNA fragment with an XbaI sticky end and an NdeI site at the other end. The PelB upper and lower anneal to also have XbaI and NdeI sticky ends. There is a ribosome binding site,
, and a spacer region, TAAATA, included between the XbaI restriction site and the start codon
, and a spacer region, TAAATA, included between the XbaI restriction site and the start codon