Table 1 Constructs used within this study
From: A cell engineering approach to enzyme-based fed-batch fermentation
| Plasmid | Description | References |
|---|---|---|
| pET29a(+) | T7 promoter, Kanr, ability for C-terminal 6-His tag | Novagen |
| pQR1344 | pET29a containing eGFP, T7 promoter, Kanr | D. Dobrijevic (unpublished) |
| pQR1703 | pET29a containing D. geothermalis glucoamylase, T7 promoter, Kanr | This study |
| pQR1704 | pET29a containing T. tengcongensis glucoamylase, T7 promoter, Kanr | This study |
| pQR1705 | pET29a containing C. violaceum glucoamylase, T7 promoter, Kanr | This study |
| pET28a(+) | T7 promoter, Kanr, ability for N-terminal 6-His tag | Novagen |
| pQR1706 | pET28a containing D. geothermalis glucoamylase, T7 promoter, Kanr | This study |
| pQR1707 | pET28a containing T. tengcongensis glucoamylase, T7 promoter, Kanr | This study |
| pQR1708 | pET28a containing C. violaceum glucoamylase, T7 promoter, Kanr | This study |
| pQR1709 | pQR1703 with DsbA signal sequence, T7 promoter, Kanr | This study |
| pQR1710 | pQR1703 with PelB signal sequence, T7 promoter, Kanr | This study |
| pQR1711 | pQR1705 lacking native signal sequence, T7 promoter, Kanr | This study |
| pQR1712 | pQR1711 with DsbA signal sequence, T7 promoter, Kanr | This study |
| BBa_K118011 | pSB1C3 containing PcstA, Camr | iGEM 2016 Distribution Kit |
| pQR1715 | BBa_K118011 containing C. violaceum glucoamylase with attached DsbA signal sequence, PcstA, Camr | This study |
| pUC19 | lac promoter, Ampr | Yanisch-Perron et al. [31] |
| pQR1720 | pUC19 containing eGFP followed by C. violaceum glucoamylase with DsbA signal sequence, lac promoter, Ampr | This study |