Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei

Table 1 Purification scheme of recombinantly produced PcCDH_Tr

	Volume	Volumetric activity^a	Protein conc	Total activity^a	Total protein	Specific activity^a	RZ	Yield	Purification factor
	[mL]	[U mL⁻¹]	[mg mL⁻¹]	[U]	[mg]	[U mg⁻¹]	[A₄₂₀/A₂₈₀]	[%]	[fold]
Supernatant (filtered)	1250	0.61	0.15^b	765	186^b	4.11^b	0.14	100	1
After HIC (PHE Source)	600	1.12	0.26^b	670	156^b	4.29^b	0.37	88	1.04
After AIEX (Source Q)	24	30.38	4.7^b	729	113^b	6.46^b	0.62	95	1.57
			2.4^c		58^c	12.7^c

^aActivity measured with lactose and cytochrome c at pH 4.5
^bProtein concentration measured with Bradford assay
^cProtein concentration determined via the absorbance at 280 nm and ε₂₈₀ = 156,400 M⁻¹ cm⁻¹

ISSN: 1475-2859