Figure 3

Quantification of PHB synthesis in the cytosol of P. tricornutum. The level of PHB synthesis for five transgenic P. tricornutum cell lines (No. 8, 11, 15, 18, 28) was analyzed by gas chromatography coupled to mass spectrometry. After 7 days of PhaA/PhaB/PhaC expression PHB levels of 8.0 to 10.6% of algal dry weight were detected. Wild type cells were negative for PHB synthesis. dwt - dry weight, wt - wild type